SIRT2 Inhibitor Screening Assay Kit (Fluorometric)

Catalog number
K322-100
Name
SIRT2 Inhibitor Screening Assay Kit (Fluorometric)
Size
100 assays
Price
581.00 EUR
Supplier

Details

Kit's description
Sirtuin or Sir2 proteins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins have been implicated in influencing aging and regulating transcription, apoptosis and stress resistance, as well as energy efficiency and alertness during low-calorie situations. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction couples lysine deacetylation to NAD hydrolysis. This hydrolysis yields O-acetyl-ADP-ribose, the deacetylated substrate and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. In BioVision’s Sirtuin inhibitor screening Kit, Sirtuin deacetylates the substrate and then the developer cleaves the deacetylated substrate to release the fluorescent group, which can be detected fluorometrically at Ex/Em = 395/541 nm. In the presence of SIRT inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high-throughput screening of SIRT2 inhibitors. Inhibitor control (Nicotinamide) is included to compare the efficacy of the test inhibitors.
Highlights
• Detection method: Fluorescence (Ex/Em 395/541 nm) • Species reactivity: Mammalian • Application: • Screening and characterization of Sirtuin inhibitors.
Kit's benefits
• Simple procedure • Fast and convenient • The assay is sensitive, reliable, and high-throughput adaptable.
Category
Epigenetics, Sirtuin 2
Kit's other name
Sirtuin 2 Inhibitor Screening Assay Kit, SIR2-like protein 2 Inhibitor Screening Assay Kit, NAD-dependent protein deacetylase sirtuin-2 Inhibitor Screening Assay Kit
Contents
• Assay Buffer • Substrate • Cofactor • Developer • SIRT2 Enzyme • Inhibitor Control (Nicotinamide)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
N/A
Test
Biovision supplies other types of Assays as 1.
Description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.