AHCY Inhibitor Screening Kit (Fluorometric)

Adenosyl homocysteinase (AHCY) (EC 3.3.1.1) or S-adenosyl homocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies). In BioVision’s AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.

• Detection method: Fluorescence (Ex/Em = 392/482 nm) • Application:Screening/characterizing/studying AHCY inhibitors

• Simple and reliable test to screen AHCY inhibitors • High-throughput suitable • Includes Inhibitor Control (3-Deazaneplanocin A)

Adenosylhomocysteinase, AHCY, S-adenosylhomocysteine hydrolase, SAHH

S-adenosylhomocysteine hydrolase (SAHH) Inhibitor Screening Kit (Fluorometric), S-adenosylhomocysteine synthase Inhibitor Screening Kit (Fluorometric), Adenosylhomocysteine hydrolase Inhibitor Screening Kit (Fluorometric)

• AHCY Assay Buffer • AHCY Reconstitution Buffer • AHCY Enzyme • Adenosine Deaminase • 3-Deazaneplanocin A (10 µM) (in DMSO) • AHCY Substrate (in DMSO) • Thiol Detecting Reagent (in DMSO)

-20°C

gel pack

12 months

see datasheet

Screening/characterizing/studying AHCY inhibitors

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.