NAD/NADH Quantitation Colorimetric Kit

Catalog number
K337-100
Name
NAD/NADH Quantitation Colorimetric Kit
Size
100 assays
Price
581.00 EUR
Supplier

Details

Kit's description
Assay of nicotinamide nucleotides is of continual interest in the studies of energy transforming and redox state of cells or tissues. BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio. The NAD Cycling Enzyme Mix in the kit specifically recognizes NADH/NAD in an enzyme cycling reaction. There is no requirement to purify NADH/NAD from samples. The reaction specifically detects NADH and NAD, but not NADP nor NADPH. The enzyme cycling reaction significantly increases the detection sensitivity and specificity. NADt (NAD and NADH) or NADH can be easily quantified by comparing with standard NADH.
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.
Kit's benefits
• Simple procedure; takes ~2 hours • Fast and convenient • Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.
Category
NAD/NADH
Kit's other name
none
Contents
• NADH/NAD Extraction Buffer • NAD Cycling Buffer • NAD Cycling Enzyme Mix • NADH Developer • Stop Solution • NADH Standard (MW:763)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.