InSitu HDAC Activity Fluorometric Assay Kit

Catalog number
K339-100
Name
InSitu HDAC Activity Fluorometric Assay Kit
Size
100 assays
Price
449.00 EUR
Supplier

Details

Kit's description
Histone acetylases (HAT’s) and Histone deacetylases (HDAC’s) are associated with regulation of gene expression. In general, increased levels of histone acetylation are associated with increased transcriptional activity, whereas decreased levels of acetylation are associated with repression of gene expression. HDAC’s are localized in both the cytosol and nucleus and some shuttle between the nucleus and cytosol. Increased HDAC expression has been observed in various cancers. BioVision’s InSitu HDAC Activity Fluorometric Assay Kit provides a direct, fast, fluorescence-based method to measure the InSitu HDAC activity. The procedure requires two easy steps, all performed in the same cell culture plate. First, the cell permeable HDAC Substrate, which comprises an acetylated lysine side chain, is incubated with cells grown in a 96-well plate. Inside the cells, HDAC deacetylates the substrate. The second step involves lysing the cells and treating with the Developer that produces a fluorophore from the Deacetylated HDAC Substrate. The generated fluorescence can be quantified at Ex/Em = 368/442 nm. The assay is well suited for high throughput screening applications.
Highlights
• Detection method: Fluorescence (Ex/Em = 368/442 nm) • Application: : Measurement of HDAC activity in cells in 96:well format : Screening HDAC inhibitors or activators : Studying growth factors or other regulators that influence HDAC activity
Kit's benefits
• Simple procedure; just requires two easy steps • Direct, Fast, Fluorescence based • Kit contains all necessary reagents for measuring InSitu HDAC activity in adherent or suspension cells
Category
HDAC
Kit's other name
none
Contents
• HDAC Assay Buffer • HDAC Substrate • Developer • HDAC Inhibitor (Trichostatin A [TSA], 1 mM) • Positive Control (Jurkat Cell Lysate) • Standard (Deacetylated Substrate, 4 mM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
• Cultured adherent or suspension cells
Test
Biovision supplies other types of Assays as 1.