Beta-Secretase Activity Fluorometric Assay Kit

β-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer’s disease. The β-Secretase activity Assay Kit provides a convenient fluorescence method for detecting β-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.

• Detection method: Fluorescence (Ex/Em 335:355/ 495:510 nm) • Species reactivity: Mammalian • Applications: Convenient fluorescence method for detecting β:secretase activity in biological and purified samples.

• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable.

Secretase

BACE1 Activity Fluorometric Assay Kit, ASP2 Activity Fluorometric Assay Kit, Memapsin-2 Activity Fluorometric Assay Kit

• β-Secretase Extraction Buffer • β-Secretase Reaction Buffer (2X) • β-Secretase Substrate (in DMSO) • Active β-Secretase (Lyophilized) • β-Secretase Inhibitor (in DMSO)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.