Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit

Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. BioVision has developed an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The assay can detect 0.1 to 10 nmol of CoA (2.5:250 µM concentration range) in a variety of samples.

• Simple procedure; takes less than 1 hour to complete the assay • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable

Coenzyme

none

• CoA Assay Buffer • OxiRed Probe • DMSO (anhydrous) • Conversion Enzyme Mix • CoA Substrate • Acyl CoA Developer • CoA Standard (10 µmol)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.