Iron Colorimetric Assay Kit

Catalog number
K390-100
Name
Iron Colorimetric Assay Kit
Size
100 assays
Price
510.00 EUR
Supplier

Details

Kit's description
Iron is essential to nearly all known organisms. It is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Inorganic iron also contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase and hydrogenase. BioVision's Iron Assay Kit provides a simple convenient means of measuring Ferrous and/or Ferric ion in sample. In the assay, ferric carrier protein will dissociate ferric into solution in the presence of acid buffer. After reduction to the ferrous form (Fe²ᶧ), iron reacts with Ferene S to produce a stable colored complex and give absorbance at 593 nm. A specific chelate chemical is included in the buffer to block copper ion (Cu²ᶧ) interference. The kit measures iron in the linear range of 0.4 to 20 nmol in 50 µl sample, or 8 µM to 400 µM iron concentration in various samples.
Highlights
• Detection method: Absorbance (593 nm) • Species reactivity: Mammalian • Applications: The kit can detect 8 µM to 400 µM iron concentration in various samples.
Kit's benefits
• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • A specific chelate chemical is included in the buffer to block copper ion (Cu²ᶧ) interference.
Category
Iron Assay
Kit's other name
none
Contents
• Iron Assay Buffer • Iron Probe • Iron Reducer • Iron Standard (100 mM)
Storage condition
+4°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue lysates, culture media, urine, and serum, as well as many other biological fluids
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.