ApoBrdU Red DNA Fragmentation Kit

Catalog number
K404-60
Name
ApoBrdU Red DNA Fragmentation Kit
Size
60 assays
Price
702.00 EUR
Supplier

Details

Kit's description
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-Red In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a Red fluorescence labeled anti-BrdU monoclonal antibody. The assay is suitable for studying apoptosis with GFP transfected cells.
Highlights
• Detection method: Flow cytometry (Ex/Em = 488/576 nm for BrdU:Red and 488/655 nm for 7:AAD). • Species reactivity: Mammalian • Applications: The TUNEL:based immunohistochemical staining kit provides complete reagents including positive and negative control cells for convenient detection of DNA fragmentation
Kit's benefits
• Simple procedure; takes ~ 3 hours • Fast and convenient • The assay is sensitive and stable • The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Category
TUNEL Assay
Kit's other name
none
Contents
• Positive Control Cells • Negative Control Cells • Wash Buffer • Reaction Buffer • TdT Enzymes • Br-dUTP • Rinse Buffer • Anti-BrdU-Red Antibody • 7-AAD/RNase Staining Buffer
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Tissue sections and fixed cell preparations
Conjugation
red dye