Alkaline Phosphatase Activity Colorimetric Assay Kit
Catalog number
K412-500
Name
Alkaline Phosphatase Activity Colorimetric Assay Kit
Size
500 assays
Price
500.00 EUR
Supplier
Details
Kit's description
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone. BioVision’s Alkaline Phosphatase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure ALP activity in serum and biological samples. It contains 10 substrate tablets providing convenience for multiple usages. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax= 405 nm) when dephosphorylated by ALP. The Kit can detect 10-250 µU ALP in samples.
Highlights
• Detection method: Absorbance (405 nm) • Species reactivity: Mammalian • Applications: The Kit can detect 10:250 µU ALP in samples.
Kit's benefits
• The assay detects alkaline phosphate (ALP) activity conveniently in various biological samples
Category
Phosphate assay
Kit's other name
none
Contents
• ALP Assay Buffer • pNPP (10 TAB) • ALP Enzyme • Stop Solution
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.