Phosphate Assay Kit (Fluorometric)

Catalog number
K420-100
Name
Phosphate Assay Kit (Fluorometric)
Size
100 assays
Price
348.00 EUR
Supplier

Details

Kit's description
Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of its most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in a variety of samples. The newly designed and enhanced Phosphate Assay Kit provides a sensitive, easy and quick means of assessing Pi over a wide range of concentrations. In this assay, inorganic phosphate reacts with sucrose to produce glucose-1-phosphate in the presence of a proprietary enzyme. The glucose-1-phosphate is specifically oxidized to generate a product that reacts with the PicoProbe™ to generate fluorescence (Ex/Em = 535/587 nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5’-nucleotidase, protein phosphatases, acid and alkaline phosphatases, phosphorylase, etc. from a variety of samples. Unlike other commercially available assays, this assay is not affected by the presence of glucose in samples. This kit can determine phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.
Highlights
• Detection method: Fluorescence (Ex/Em 535/587nm) • Species reactivity: Mammalian • Applications: Measure Phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.
Kit's benefits
• Simple procedure; takes only ~2 hours • Fast and convenient • The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5’-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples.
Category
Phosphate assay
Kit's other name
none
Contents
Phosphate Assay Buffer PicoProbe™ (in DMSO) Converter Developer Phosphate Substrate Phosphate Standard (100 mM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Mammalian samples
Applications
Measure Phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.
Samples tested
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids, algal blooms and water from run-off areas of high fertilizer use
Test
Biovision supplies other types of Assays as 1.