Chloride Colorimetric Assay Kit

Catalog number
Chloride Colorimetric Assay Kit
100 assays
348.00 EUR


Kit's description
Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter. BioVision’s Chloride Assay Kit provides a quick, simple method for quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water. The assay is based upon the competition of Hg²ᶧ and Fe²ᶧ for TPTZ. The preferred Hg-TPTZ adduct exhibits no color. In the presence of Chloride, Hg²ᶧ forms HgCL2 freeing up TPTZ which then binds the available Fe²ᶧ giving a very intense absorbance with a λmax~ 620nm. The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
• Detection method: Absorbance (620 nm) • Species reactivity: Mammalian • Applications: The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
Kit's benefits
• Simple procedure; takes less than ~20 minutes • Fast and convenient •Mn²ᶧ, Cu²ᶧ, Zn²ᶧ do not interfere with the assay.
Kit's other name
• Chloride Reagent • Chloride Standard (10 µmol)
Storage condition
Shipping condition
Maximum time can be stored
12 months
Species reactivity
see datasheet
Samples tested
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
Biovision supplies other types of Assays as 1.
Colorimetric assays or detection use UV absorption or enzymatic color reaction.