Glutamine Colorimetric Assay Kit

Catalog number
K556-100
Name
Glutamine Colorimetric Assay Kit
Size
100 assays
Price
540.00 EUR
Supplier

Details

Kit's description
Glutamine (Gln) is one of the most abundant amino acids containing an uncharged amide as a side chain. It is synthesized via condensation of glutamate and ammonia. Gln is classified as a non-essential amino acid, however, Gln is important in several biological processes such as protein synthesis, regulation of acid-balance in mammalian kidneys and cell growth. It constitutes cell’s main source of nitrogen for the synthesis of nucleotides and hexosamines. Glutamine-rich diets benefit patients suffering from Crohn’s disease, severe burns, HIV/AIDS and cancer. BioVision’s Glutamine Colorimetric Assay kit is a simple and sensitive assay that detects the biologically relevant concentrations of Gln in various fluids and tissues. The assay is based on the hydrolysis of Glutamine to Glutamate producing a stable signal, which is directly proportional to the amount of Gln in the sample. The assay can detect as little as 25 µM of Gln in a variety of biological samples.
Highlights
• Detection method: Absorbance (450 nm) • Applications: Measurement of Glutamine in various biological samples
Kit's benefits
• Convenient & Rapid Protocol • This kit can detect as little as 25 µM of Glutamine in a variety of biological samples
Category
Glutamine
Kit's other name
none
Contents
• Hydrolysis Buffer • Development Buffer • Hydrolysis Enzyme Mix (Lyophilized) • Development Enzyme Mix (Lyophilized) • Developer (Lyophilized) • Gln Standard (Lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Measurement of Glutamine in various biological samples
Samples tested
• Serum, plasma, urine or other biological fluids • Mammalian tissues: kidney, liver, brain samples etc.
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.