Sialic Acid (NANA) Colorimetric/Fluorometric Assay Kit

Catalog number
K566-100
Name
Sialic Acid (NANA) Colorimetric/Fluorometric Assay Kit
Size
100 assays
Price
510.00 EUR
Supplier

Details

Kit's description
Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid. Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been shown recently that sialic acid level may be associated with developmental and pathological stages. BioVision's Sialic Acid Assay Kit provides a simple and convenient means of measuring free Sialic Acid in a variety of biological samples. The kit utilizes an enzyme coupled reaction in which free sialic acid is oxidized resulting in development of the Oxi-Red probe to give fluorescence (Ex/Em=535/587 nm) and absorbance (O.D.=570 nm). The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 µM concentration.
Highlights
• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 µM concentration.
Kit's benefits
• Simple procedure; takes less than ~40 minutes • Fast and convenient • The kit utilizes an enzyme coupled reaction in which free sialic acid is oxidized resulting in development of the Oxi-Red probe to give color
Kit's other name
none
Contents
• Sialic Acid Assay Buffer • Sialic Acid Probe in DMSO • Sialic Acid Converting Enzyme • Sialic Acid Development Mix • Sialic Acid Standard (10 µmol)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.