Phenylalanine Fluorometric Assay Kit

L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences. BioVision’s Phenylalanine Assay Kit provides a quick, simple, accurate method for quantifying PHE in biological samples. In the assay, PHE is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.

• Detection method: Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The assay is linear in the range from 0.1 to 1.0 nmol (2:20 µM) of Phenylalanine.

• Simple procedure; takes less than ~30 minutes • Fast and convenient

none

• Phenylalanine Assay Buffer • Tyrosinase • Enzyme Mix • Developer • Phenyalanine Standard (10.0 µmol)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.