Preadipocyte Isolation Kit

Catalog number
K583-5
Name
Preadipocyte Isolation Kit
Size
For 5 g Tissue
Price
510.00 EUR
Supplier

Details

Kit's description
Adipose tissue is loose connective tissue that accumulates in animals and serves primarily for energy storage, insulation, and thermoregulation. It is predominantly composed of adipocytes, but also contains a stromal vascular fraction consisting of preadipocytes, fibroblasts, vascular endothelial cells, and immune cells. Recent research has discovered a dynamic role of adipose tissue in the production and secretion of various hormones and as an active endocrine organ. The excessive accumulation of adipose is known as obesity. Ways to prevent over-accumulation of adipose is a field of intense research. In addition to obesity, adipose tissue has been found to participate in various physiological processes, including reproduction, angiogenesis, inflammation, cancer, and vascular homeostasis. BioVision’s Preadipocyte Isolation Kit provides the reagents and tools to isolate the stromal vascular fraction from adipose tissue. The resulting stromal vascular fraction is cultured on a tissue-culture plate, where preadipocytes adhere. The preadipocytes retain the ability to proliferate and differentiate into adipocytes when treated with differentiation-inducing components (Cat. # K579, 3T3-L1 Differentiation Kit). The preadipocytes can be used to study the process of adipogenesis and the differentiated adipocytes can be used to study lipolysis, endocrine activity, cell-signaling, and metabolic dysfunction.
Highlights
Application: • Isolation of preadipocytes from adipose tissue
Kit's benefits
• Simple, rapid & convenient method to isolate preadipocytes from adipose tissue.
Category
Preadipocyte
Kit's other name
none
Contents
• Collagenase (0.2%) • Collagenase Stop Buffer • Red Blood Cell Lysis Buffer • PBS • Cell Strainer (100 µm) • Cell Strainer (70 µm)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Isolation of preadipocytes from adipose tissue
Samples tested
• Animal tissues: mouse or rat adipose tissue