HMG-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric)

Catalog number
K588-100
Name
HMG-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric)
Size
100 assays
Price
616.00 EUR
Supplier

Details

Kit's description
HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol. BioVision’s HMG-CoA Reductase Activity Assay Kit measures utilization of NADPH, which can be measured by the decrease of absorbance (OD 340 nm). The kit is suitable for measuring activity of purified enzyme and screening inhibitors/activators of HMG-CoA reductase. The limit of detection is below 0.05 mU.
Highlights
• Detection method: Absorbance (OD 340 nm) • Applications: Measurement of HMG:CoA reductase activity : Screening/studying/characterizing HMG:CoA reductase inhibitors/activators
Kit's benefits
• Simple, rapid & convenient • Limit of detection: below 0.05 mU • Includes inhibitor (Atorvastatin)
Category
Lipid Metabolism
Kit's other name
HMGR Activity/Inhibitor Screening Kit (Colorimetric), 3-hydroxy-3-methyl-glutaryl-CoA reductase Activity/Inhibitor Screening Kit (Colorimetric)
Contents
• HMG-CoA Reductase Assay Buffer • HMG-CoA Reductase • HMG-CoA • NADPH • Inhibitor (Atorvastatin, 10 mM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of HMG-CoA reductase activity • Screening/studying/characterizing HMG-CoA reductase inhibitors/activators
Samples tested
• Partially purified and recombinant enzyme
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
Description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.