Fumarase Activity Colorimetric Assay Kit

Catalog number
K596-100
Name
Fumarase Activity Colorimetric Assay Kit
Size
100 assays
Price
601.00 EUR
Supplier

Details

Kit's description
Fumarase (Fumarate Hydratase: EC 4.2.1.2) is an enzyme that catalyzes the reversible reaction of hydration and dehydration of fumarate to Malate. It has two forms: mitochondrial and cytosolic forms. The mitochondrial form of fumarase is one of the key enzymes in citric acid cycle, while cytosolic form is important for metabolism of amino acids and fumarate. In humans, fumarase deficiency leads to serious health problems such as fetal brain abnormality, hypotonia, and renal cell carcinoma. Therefore, accurate measurement of fumarase activity is important for preventing, diagnosis and mechanistic study of fumarase deficiency. Biovision’s Fumarase Activity Assay kit provides a quick and easy method for monitoring fumarase activity in various samples. In this kit, fumarase converts fumarate into malate, which then reacts with Enzyme Mix to form an intermediate. The intermediate subsequently reduces the Developer to a colored product with strong absorbance at 450 nm. The assay is simple and high-throughput ready and can detect less than 50 U/ml of fumarase activity in variety of sample types.
Highlights
• Detection method: Absorbance (OD 450 nm) • Applications: : Measurement of fumarase activity in various tissues/cells. : Analysis of citric acid cycle, reductive carboxylation cycle (CO2 fixation) and renal cell carcinomas.
Kit's benefits
• Simple & Rapid Protocol • High-Throughput • This kit can detect less than 50 U/ml of fumarase activity in variety of sample types
Category
Fumarase
Kit's other name
Fumarate Hydratase Colorimetric Assay Kit
Contents
• Fumarase Assay Buffer • Fumarase Substrate • Fumarase Enzyme Mix (Lyophilized) • Fumarase Developer (Lyophilized) • NADH Standard (Lyophilized) • Fumarase Positive Control (Lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of fumarase activity in various tissues/cells. • Analysis of citric acid cycle, reductive carboxylation cycle (CO2 fixation) and renal cell carcinomas.
Samples tested
• Animal tissues: liver, heart, muscle etc. • Isolated mitochondria. • Cell culture: Adherent or suspension cells.
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.