Succinyl-CoA Synthetase Activity Colorimetric Assay Kit

Catalog number
K597-100
Name
Succinyl-CoA Synthetase Activity Colorimetric Assay Kit
Size
100 assays
Price
732.00 EUR
Supplier

Details

Kit's description
Succinyl-CoA Synthetase (SCS, also called Succinyl-CoA ligase, Succinate Thiokinase) (EC 6.2.1.5) is a critical enzyme in the citric acid cycle and an important metabolic intermediate for porphyrin, heme and ketone body biosynthesis. It is located in the mitochondrial matrix and is a heterodimer composed of one α and one β subunit. In humans, Succinyl-CoA Synthetase deficiency causes the build-up of lactic acid leading to lactic acidosis, which can be fatal in infants. Measurement and analysis of SCS activity is useful for both mechanistic studies as well as for diagnostic purposes. In BioVision’s Succinyl-CoA Synthetase Activity Assay, SCS converts succinate into succinyl-CoA in the presence of ATP and CoA. Succinyl-CoA reacts with the Developer to form a colored product with strong absorbance at 450 nm. This assay kit is simple, sensitive, and high-throughput adaptable. It can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.
Highlights
• Detection method: Absorbance (OD 450 nm). • Applications: • Measurement of Succinyl:CoA Synthetase activity in various tissues/cells • Analysis of cell signaling pathway
Kit's benefits
• Simple & Rapid Protocol • High-Throughput adaptable • This kit can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples.
Category
Succinyl-CoA Synthetase
Kit's other name
none
Contents
• SCS Assay Buffer • SCS Substrate Mix (Lyophilized) • SCS Enzyme Mix (Lyophilized) • SCS Developer (Lyophilized) • NADH Standard (Lyophilized) • SCS Positive Control (Lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of Succinyl-CoA Synthetase activity in various tissues/cells • Analysis of cell signaling pathway
Samples tested
• Animal tissues: heart, liver, muscle, etc. • Purified mitochondria • Cell culture: adherent or suspension cells
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.