Sphingomyelinase Activity Colorimetric Assay Kit

Catalog number
K599-100
Name
Sphingomyelinase Activity Colorimetric Assay Kit
Size
100 assays
Price
429.00 EUR
Supplier

Details

Kit's description
Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation, and sterol homeostasis. There are five distinct types of SMases. BioVision’s SMase Activity Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using colorimetry (OD 570 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce an intermediate that reacts with a highly specific probe to generate color. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 1.25 mU/ml (~5 µU/mg tissue/cells) in a variety of samples.
Highlights
• Detection method: Absorbance (OD 570 nm) • Applications: : Measurement of SMase activity in various tissues/cells extracts : Screening of SMase inhibitors
Kit's benefits
• Simple, rapid & convenient • The assay can detect sphingomyleinase activity as low as 1.25 mU/ml (~5 µU/mg tissue/cells) in a variety of samples
Category
Sphingomyleinase
Kit's other name
none
Contents
• SMase Assay Buffer • SMase Probe (DMSO) • SMase Substrate (Lyophilized) • SMase Enzyme Mix I (Lyophilized) • SMase Enzyme Mix II (Lyophilized) • Choline Standard (Lyophilized) • SMase Positive Control (Lyophilized) • SMase Extraction Detergent
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of SMase activity in various tissues/cells extracts • Screening of SMase inhibitors
Samples tested
• Animal tissues: brain, heart, kidney, etc. • Cell culture: Adherent or suspension cells
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.