Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit

Catalog number
K603-100
Name
Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit
Size
100 assays
Price
510.00 EUR
Supplier

Details

Kit's description
The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both by colorimetric or fluorometric methods. Majority of the cholesterol in blood is in the form of cholesteryl esters which can be hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield H₂O₂which reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters).
Highlights
• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Application: The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction.
Kit's benefits
• Simple procedure; takes ~60 minutes • Fast and convenient
Category
Cholesterol Assay
Kit's other name
none
Contents
• Cholesterol Assay Buffer • Cholesterol Probe (in DMSO, anhydrous) • Enzyme Mix (lyophilized) • Cholesterol Esterase (lyophilized) • Cholesterol Standard (2 µg/µl)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
Tissue
total
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.