Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit

Choline and acetylcholine play important roles in many biological processes. BioVision’s Choline/Acetylcholine Quantification Kit provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method. In the assay free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm). Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction. The kit can detect choline and acetylcholine (total choline – free choline) in various biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect 10 pmol~5 nmol of choline or acetylcholine.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Application: The kit can detect 10 pmol to ~5 nmol of choline or acetylcholine.

• Simple procedure; takes ~40 minutes • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of Choline/Acetylcholine in various biological samples

Choline/Acetylcholine

none

• Choline Assay Buffer • Choline Probe • Choline Enzyme Mix • Acetylcholinesterase • Choline Standard (5 µmol)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Colorimetric assays or detection use UV absorption or enzymatic color reaction.