Triglyceride Quantification Colorimetric/Fluorometric Kit

Triglycerides (TG) are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. TG are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. Theglycerol is then oxidized to generate a product which reacts with theprobe to generate color (spectrophotometry at λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The kit can detect 2 pmol:10nmol (or 2:10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.

• Simple procedure; takes ~1 hour • Fast and convenient • Kit contains the necessary reagents for accurate measurement of triglyceride in various biological samples

Triglyceride

none

• Triglyceride Assay Buffer • TriglycerideProbe (in DMSO, anhydrous) • Lipase • TriglycerideEnzyme Mix (lyophilized) • Triglyceride Standard (1 mM)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Colorimetric assays or detection use UV absorption or enzymatic color reaction.