Sucrose Colorimetric/Fluorometric Assay Kit

Catalog number
K626-100
Name
Sucrose Colorimetric/Fluorometric Assay Kit
Size
100 assays
Price
480.00 EUR
Supplier

Details

Kit's description
Sucrose (C₁₂H₂₂O₁₁; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose. BioVision’s Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.
Highlights
• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The method can detect 0.0002:10 mM sucrose concentrations.
Kit's benefits
• Simple procedure; takes ~1 hour • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Sucrose
Category
Sucrose
Kit's other name
none
Contents
• Sucrose Assay Buffer • OxiRed™ Probe (in DMSO) • Invertase (Lyophilized) • Enzyme Mix (Lyophilized) • Sucrose Standard (100 nmol/µl)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring level during fermentation and feeding in protein expression processes
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.