PicoProbe™ β-Hydroxybutyrate Fluorometric Assay Kit

Catalog number
K651-100
Name
PicoProbe™ β-Hydroxybutyrate Fluorometric Assay Kit
Size
100 assays
Price
510.00 EUR
Supplier

Details

Kit's description
β-Hydroxybutyrate (β-HB) is the most abundant of the ketone bodies (~78% of total ketone bodies in blood). Ketoacidosis (high concentrations of the ketone bodies) can be caused by a variety of conditions, such as diabetes, alcoholism and severe starvation. Ketoacidosis can be fatal if not treated. Additionally, ketogenic diets can increase the concentration of ketone bodies slightly in healthy individuals. BioVision’s β-Hydroxybutyrate Assay Kit offers simplicity, enhanced sensitivity, and can be adapted to high-throughput applications. The assay is based on enzymatic oxidation of β-HB that results in generation of fluorescent signal (Ex/Em = 535/587 nm) which is directly proportional to the amount of β-HB. The kit offers an excellent alternative for measurement of β-HB when biological sample quantities are limited or subketogenic levels are suspected. The assay can detect β-Hydroxybutyrate as low as 4 µM in a variety of biological samples.
Highlights
• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Applications: : Measurement of β:Hydroxybutyrate in various biological samples : Analysis of β:Hydroxybutyrate in pathological conditions
Kit's benefits
• Simple, rapid & high-throughput adaptable • Highly sensitive: The assay can detect less than 4 µM of β-Hydroxybutyrate (β-HB) in various sample types.
Category
Beta-hydroxybutyrate
Kit's other name
PicoProbe™ 3-Hydroxybutyrate Fluorometric Assay Kit
Contents
• β-HB Assay Buffer • PicoProbe™ (in DMSO) • β-HB Enzyme Mix (Lyophilized) • β-HB Substrate Mix (Lyophilized) • β-HB Standard (Lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of β-Hydroxybutyrate in various biological samples • Analysis of β-Hydroxybutyrate in pathological conditions
Samples tested
• Serum, plasma • Urine or other body fluids • Food such as milk etc.
Test
Biovision supplies other types of Assays as 1.
Conjugate
Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit