Ascorbic Acid Colorimetric/Fluorometric Assay Kit

Catalog number
K661-100
Name
Ascorbic Acid Colorimetric/Fluorometric Assay Kit
Size
100 assays
Price
449.00 EUR
Supplier

Details

Kit's description
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples. BioVision’s Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.
Highlights
• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The assay can detect 0.01:10 nmol of ascorbic acid per assay in various samples.
Kit's benefits
• Simple procedure; takes ~ 5 minutes for the reaction to complete • Fast and convenient
Category
Ascorbic Acid Assay
Kit's other name
none
Contents
• Ascorbic Acid Assay Buffer • Ascorbic Acid Probe (in DMSO) • Catalyst • Ascorbic Acid Enzyme Mix (lyophilized) • Ascorbic Acid Standard (20 µmole)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, food samples, growth medium, etc.
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.