Oxalate Decarboxylase Activity Colorimetric Assay Kit

Oxalate Decarboxylase (OXDC, EC 4.1.1.2) belongs to the cupin superfamily and is composed of two β-barrel domains. OXDC catalyzes the conversion of Oxalate into Formate and CO2 and plays an important role in stress response. In humans, high levels of oxalate can lead to various health problems including hyperoxaluria, kidney stones, and renal failure. Wood rotting fungi generates high levels of oxalate, causing rot in many crops including lettuce, soybean, dry bean, and tomato etc. Recent studies show that overexpression of OXDC in plants (e.g. tomato, soybean, lettuce, and tobacco) results in transgenic plants with resistance to fungal pathogenesis. Accurate measurement of Oxalate Decarboxylase activity is useful for a variety of therapeutic, diagnostic, and mechanistic studies. BioVision’s Oxalate Decarboxylase Activity Assay kit provides a quick and easy way to measure Oxalate Decarboxylase activity in various samples. In this assay, Oxalate Decarboxylase converts oxalate to formate, which subsequently converts a nearly colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, high-throughput adaptable and can detect less than 20 µU of oxalate decarboxylase activity in a variety of samples.

• Detection method: Absorbance (OD 450 nm) • Applications: : Measurement of Oxalate Decarboxylase activity in various plant tissues : Analysis of cell signaling pathways such as glyoxylate and dicarboxylate metabolism

• Simple, convenient and high throughput adaptable • Kit can detect Oxalate decarboxylase activity less than 20 µU.

Oxalate decarboxylase

none

• OXDC Assay Buffer I • OXDC Assay Buffer II • OXDC Substrate (Lyophilized) • OXDC Enzyme Mix (Lyophilized) • OXDC Probe (Lyophilized) • OXDC Positive Control • Formate Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

• Measurement of Oxalate Decarboxylase activity in various plant tissues • Analysis of cell signaling pathways such as glyoxylate and dicarboxylate metabolism

• Fungi such as mushroom • Plant tissues

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.