Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit

Triose Phosphate Isomerase (TPI or TIM: EC 5.3.1.1) is an important enzyme for glycolysis. It reversibly interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms. In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment. BioVision’s Triose Phosphate Isomerase Activity Assay kit provides a quick and easy way for monitoring Triose Phosphate Isomerase activity in a variety of samples. In this kit, Triose Phosphate Isomerase converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the Enzyme Mix & Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, & high-throughput and can detect Triose Phosphate Isomerase activity as low as 40 mU/ml.

• Detection method: Absorbance (OD 450 nm) • Applications: : Measurement of TPI activity in various tissues and cells : Analysis of glycolysis & pentose phosphate pathway and lipid metabolism

• Simple, highly sensitive, and high-throughput. • The assay can measure Triose Phosphate Isomerase activity as low as 40 mU/ml.

Triose Phosphate Isomerase Activity

none

• TPI Assay Buffer • TPI Substrate (Lyophilized) • TPI Enzyme Mix (Lyophilized) • TPI Developer (Lyophilized) • NADH Standard (Lyophilized) • TPI Positive Control (Lyophilized)

-20°C

gel pack

12 months

see datasheet

• Measurement of TPI activity in various tissues and cells • Analysis of glycolysis & pentose phosphate pathway and lipid metabolism

• Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: Adherent or suspension Cells • Human serum or plasma

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.