Invertase Activity Colorimetric Assay Kit

Catalog number
K674-100
Name
Invertase Activity Colorimetric Assay Kit
Size
100 assays
Price
480.00 EUR
Supplier

Details

Kit's description
Invertase (EC 3.2.1.26), also known as sucrase or β -fructofuranosidase, catalyzes the hydrolysis of sucrose (table sugar) by cleaving its glycosidic bond and forming one molecule each of glucose and fructose. Invertase is widely expressed among plants and microorganisms including yeast and bacteria. It is extensively utilized in industrial processes, including food, ethanol production, cosmetics, drugs, paper and bioelectronics electrodes. BioVision’s Invertase Activity Assay kit provides a simple, sensitive and high-throughput adaptable assay that detects relevant concentrations of Invertase in biological samples or purified/crude enzyme preparations. The assay is based on the enzymatic detection of glucose, one of the hydrolysis products. Detection limit: 20 µU.
Highlights
• Detection method: Absorbance (570 nm) • Species reactivity: Bacteria, Mammals • Application : detection of Invertase activiy as low as 20 µU in various biological fluids, bacteria and crude enzyme preparations.
Kit's benefits
• Simple, Sensitive & Rapid protocol • Highly-Sensitive: Detects as low as 20 µU Invertase in various sample types
Category
Invertase Assay
Kit's other name
Saccharase activity assay kit, Sucrase activity assay kit, Beta-fructofuranosidase assay
Contents
• Invertase Hydrolysis Buffer • Invertase Assay Buffer • Invertase Stop Solution • OxiRedTM Probe (in DMSO) • Invertase Enzyme Mix • Invertase Substrate • Glucose Standard (100 mM) • Positive Control
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of Invertase activity in various biological fluids, bacteria and crude enzyme preparations. • Analysis of metabolic pathways such as glycolysis and Diabetes Obesity-related Signaling.
Samples tested
• Biological fluids e.g. saliva • Bacteria • Crude/purified enzyme preparations
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.