Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit

Catalog number
K677-100
Name
Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit
Size
100 assays
Price
500.00 EUR
Supplier

Details

Kit's description
α-Ketoglutarate (α-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing α-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. α-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. BioVision's α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples. In the assay, α-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The α-Ketoglutarate Assay Kit is useful for detecting α-ketoglutarate in the range of 0.01 to 10 nmoles.
Highlights
• Detection method:Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The α:Ketoglutarate Assay Kit is useful for detecting α:ketoglutarate in the range of 0.01 to 10 nmoles.
Kit's benefits
• Simple procedure; takes ~ 30 minutes to complete the assay • Fast and convenient • Highly sensitive assays for measuring alpha-Ketaglutarate levels in biological samples
Category
Ketoglutarate
Kit's other name
none
Contents
• α-KG Assay Buffer • α-KG OxiRed Probe (in DMSO) • α-KG Converting Enzyme • α-KG Development Enzyme Mix • α-KG Standard (10 µmol)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
Description
The Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.