Phospholipase D (PLD) Activity Colorimetric Assay Kit

Catalog number
K725-100
Name
Phospholipase D (PLD) Activity Colorimetric Assay Kit
Size
100 assays
Price
429.00 EUR
Supplier

Details

Kit's description
Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders. BioVision’s Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from Phosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples.
Highlights
• Detection method: Absorbance (OD 570 nm) • Applications: : Measurement of PLD activity in various tissues/cells : Analyzing intracellular signaling : Screening of PLD inhibitors
Kit's benefits
• Simple, rapid & convenient • The assay can detect phospholipase D (PLD) Activity less than 1 mU/ml in various sample types.
Category
Phospholipase D
Kit's other name
Lecithinase D Activity Colorimetric Assay Kit, Choline Phosphatase Activity Colorimetric Assay Kit, Lipophosphodiesterase II Activity Colorimetric Assay Kit
Contents
• PLD Assay Buffer • PLD Probe (in DMSO) • PLD Substrate • PLD Enzyme Mix (Lyophilized) • Choline Standard (Lyophilized) • PLD Positive Control (Lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of PLD activity in various tissues/cells • Analyzing intracellular signaling • Screening of PLD inhibitors
Samples tested
• Animal tissues: liver, brain, kidney, etc. • Cell culture: Adherent or suspension cells
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.