Phosphofructokinase (PFK) Activity Colorimetric Assay Kit

Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet). In humans, PFK deficiency causes glycogen storage disease, also called Tarui’s disease, which is characterized by exercise-induced muscle weakness and cramps. On the other hand, increased PFK activity contributes to cancer cell proliferation and tumorigenicity. Early detection of abnormal phosphofructokinase activity is crucial for diagnosis, prediction and therapeutic strategy. In BioVision’s Phosphofructokinase Activity Assay kit, PFK converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. PFK activity assay is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.

• Detection method : Absorbance (450 nm) • Application: The kit can detect phosphofructokinase activity less than 1 mU.

• Simple procedure • Rapid, Convenient and Reliable • Sensitive assay for measuring phosphofructokinase activity in various biological samples

Phosphofructokinase (PFK)

none

• PFK Assay Buffer • PFK Substrate (lyophilized) • ATP (lyophilized) • PFK Enzyme Mix (Lyophilized) • PFK Developer (Lyophilized) • NADH Standard (Lyophilized) • Positive Control (Lyophilized)

-20°C

gel pack

12 months

see datasheet

Inquire

• Animal tissues: Liver, Brain, Heart, Muscles etc. • Cell culture: Adherent or suspension cells.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.