Luciferase Reporter Assay Kit

Catalog number
K801-200
Name
Luciferase Reporter Assay Kit
Size
200 assays
Price
196.00 EUR
Supplier

Details

Kit's description
Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer. BioVision’s Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg²ᶧ and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash Kinetics). The luminescent reaction is then triggered by an injection of luciferin, and the emitted light is recorded by a luminometer. By providing CoA, along with ATP, Mg²ᶧ in an optimized buffer solution, BioVision’s Luciferase Reporter Assay Kit ensure maximal sensitivity, consistent light output, as well as convenience and consistency when working with multiple samples.
Highlights
• Detection method: Luminescence • Species reactivity: Mammalian • Application: This Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells.
Kit's benefits
• Simple procedure; takes ~ 20-30 minutes • Fast and convenient • The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.
Category
Luciferase
Kit's other name
none
Contents
• Substrate A • Substrate B • Cell Lysis Buffer
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture homogenates (eukaryotic cells)
Test
Biovision supplies other types of Assays as 1.