Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit

Catalog number
K807-100
Name
Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit
Size
100 assays
Price
834.00 EUR
Supplier

Details

Kit's description
Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington’s disease, while in Wilson’s disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease. BioVision’s AHCY Activity Assay kit can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the OxiRed™ Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification (L.O.Q) is 1 µU recombinant human AHCY.
Highlights
• Detection Method – Fluorescence (Ex/Em = 535/587 nm) • Application: Detection of AHCY activity
Kit's benefits
• Rapid, simple & convenient • The assay can detect AHCY activity as low as 1 µU recombinant human AHCY.
Category
Adenosylhomocysteinase, AHCY, S-adenosylhomocysteine hydrolase, SAHH
Kit's other name
S-adenosylhomocysteine hydrolase (SAHH) Activity Fluorometric Assay Kit, S-adenosylhomocysteine synthase Activity Fluorometric Assay Kit, Adenosylhomocysteine hydrolase Activity Fluorometric Assay Kit
Contents
• AHCY Assay Buffer • Homogenization Buffer • OxiRed™ Probe • SAH Substrate • Enzyme Mix I • Enzyme Mix II • Adenosine Standard (10 mM) • AHCY Enzyme (Positive Control)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Detection of AHCY activity
Samples tested
• Purified recombinant protein • Cell and tissue lysate
Test
Biovision supplies other types of Assays as 1.