SuperBrite™ ECL Western Blot Substrate/Detection Kit

Catalog number
K824-50
Name
SuperBrite™ ECL Western Blot Substrate/Detection Kit
Size
50 ml
Price
196.00 EUR
Supplier

Details

Kit's description
BioVision’s SuperBrite™ ECL kit includes second-generation ECL substrates. They are highly sensitive, non-radioactive, and enhanced luminol-based chemiluminescent substrates for easy detection of horseradish peroxidase (HRP) on immunoblots. Blots can be repeatedly exposed to X-ray film or a CCD camera to obtain optimal results or stripped off the immune-detection reagents and re-probed.
Highlights
The most sensitive ECL Western blotting substrate kit on the market. Application: Western Blotting, Dot Blotting.
Kit's benefits
50 ml SuperBrite™ ECL Western Blotting solutions, sufficient for 30 min-gel blots or 2500 cm2 of membrane III. Advantages: • Minimal volumes required: Can be used for 30 mini-gel blots or 2500 cm2 of membrane. • Exceptional Sensitivity: Detects targets in the low picogram levels. • Multiple imaging options: Compatible with X-Ray, CCD and laser based imaging. • Ready to Use: No additional reconstitutions required. • Stable signal: Signals developed are stable. • Economical: Available at the most competitive rates.
Category
Western Blotting Substrate
Kit's other name
Western blot ECL substrate kit
Contents
Reagent A Reagent B
Storage condition
+4°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
The most sensitive ECL Western blotting substrate kit on the market. Application: Western Blotting, Dot Blotting.
Applications
Western blot. Dot blot
Samples tested
pure proteins, cell/tissue extracts or lysates
Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Description
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.