EZScreen™ Triglyceride Quantification Colorimetric Assay Kit (384-well)

Catalog number
K952-400
Name
EZScreen™ Triglyceride Quantification Colorimetric Assay Kit (384-well)
Size
400 assays
Price
763.00 EUR
Supplier

Details

Kit's description
Triglycerides (TGs) are the main constituent of body fat in humans and animals, as well as vegetable fat. TGs are found as a natural component in human’s blood circulatory system. TGs are composed of one molecule of glycerol and 3 molecules of fatty acids. The metabolism of cholesterol generates fatty acids and glycerol, and both can serve as substrates in metabolic pathways to produce energy. Excess TGs can remain in the body as fatty deposits and that may lead to obesity. Elevated triglyceride levels also increase the risk for heart disease, strokes, and type 2 diabetes.  BioVision’s EZScreenTM Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In the assay, TGs are converted to free fatty acids and glycerol. The released glycerol is then oxidized to generate a product which reacts with a probe to generate a color that can be detected by a spectrophotometer at λ = 590 nm. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The kit provides a convenient means for detecting 0.5 to 5 mM Triglycerides in biological samples
Highlights
• Detection method: Absorbance (590 nm) • Species reactivity: Mammalian • Applications: The kit detects 0.5:500 µM triglyceride samples
Kit's benefits
• Simple procedure; takes ~40 minutes • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of triglyceride in various mammalian samples
Category
Lipid Metabolism
Kit's other name
none
Contents
• Triglyceride Assay Buffer • Triglyceride Probe (in DMSO, anhydrous) • Lipase (lyophilized) • Triglyceride Enzyme Mix (lyophilized) • Triglyceride Standard (1 mM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Mammalians
Applications
• Measure LDH activity in biological samples • Screen and characterize compounds that influence lactate metabolism
Samples tested
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.