Plasma Kallikrein Activity Assay Kit (Colorimetric)

Catalog number
K997-100
Name
Plasma Kallikrein Activity Assay Kit (Colorimetric)
Size
100 assays
Price
692.00 EUR
Supplier

Details

Kit's description
Plasma Prekallikrein (EC 3.4.21.34), is the glycosylated single chain zymogen precursor of the plasma serine protease Kallikrein. It circulates with kininogen and is activated by Factor XIIa to Kallikrein in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time. Elevated plasma Prekallikrein is associated with diabetes and cardiovascular disease. Plasma Kallikrein inhibitors have been proposed as drugs to manage Hereditary Angioedema. BioVision’s Plasma Kallikrein Activity Assay Kit utilizes the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader. The Plasma Kallikrein Specific Inhibitor (PKSI) selectively inhibits the ability of Plasma Kallikrein to the cleave the synthetic substrate. The kit is easy-to-use and can detect PK activity of Purified Plasma Kallikrein and Plasma Samples
Highlights
• Detection method: Colorimetric (Absorbance 405 nm) • Applications: Measurement of Plasma Kallikrein activity in plasma and purified/crude enzyme preparations
Kit's benefits
• Simple, rapid & convenient assay to measure Plasma Kallikrein • Includes Positive Control and Plasma Kallikrein and Plasma Kallikrein Inhibitors
Category
Metabolism
Kit's other name
none
Contents
• PK Assay Buffer • PK Activator • PK Substrate • Human PK • PKSI Inhibitor • pNA Standard (0.1 M)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Mammalians
Applications
Measurement of Plasma Kallikrein activity in plasma samples and purified/crude enzyme preparations
Samples tested
Plasma and Purified enzyme
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.