Fibronectin (rat) ELISA Kit

Catalog number
K3633-100
Name
Fibronectin (rat) ELISA Kit
Size
100 assays
Price
611.00 EUR
Supplier

Details

Kit's description
Fibronectin is a 430 KDa dimeric glycoprotein that exists in 2 forms - cellular and plasma fibronectin. BioVision’s rat Fibronectin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat Fibronectin is coated on a 96-well plate. Standards and test samples are added to the wells and Fibronectin present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Fibronectin is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat Fibronectin captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 15 pg/ml.
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity:rat • Application: Quantitative protein detection, establishing normal range, validation of antibody array results
Kit's benefits
• Easy, convenient and time-saving method to assay for rat Fibronectin. • Detection Range:156 pg/ml – 10,000 pg/ml. Sensitivity: < 15 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.
Category
Fibronectin ELISA kit
Kit's other name
Fibronectin (rat) ELISA Kit
Contents
• 96 wells coated with anti-rat monoclonal Fibronectin antibody, 1 Microplate • Lyophilized native rat Fibronectin standard (10 ng/vial) • Biotinylated anti-rat Fibronectin antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
rat
Applications
Application- Quantitative protein detection, establishing normal range
Samples tested
• Serum & plasma (heparin, EDTA) • Cell culture supernatants
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus