BRCA1 (Human) ELISA Kit

Breast cancer type 1 susceptibility protein (BRCA1) is an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. BioVision’s BRCA1 ELISA kit is a sandwich ELISA assay for the quantitative measurement of human BRCA1 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human BRCA1 captured from the samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of BRCA1 in human serum, plasma, tissue homogenates and other biological fluids.

Easy, convenient and time-saving method to measure the level of BRCA1 in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids. Detection Range: 0.312 – 20 ng/ml Sensitivity: < 0.188 ng/ml

ELISA kit

none

• Microplate: 96 wells • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers

4°C

gel pack

6 months

Human

Quantitative measurement of BRCA1 in human serum, plasma, tissue homogenates and other biological fluids.

Human serum, plasma, tissue homogenates and other biological fluids.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED