GDF-15 (human) ELISA Kit

Catalog number
K4569-100
Name
GDF-15 (human) ELISA Kit
Size
100 assays
Price
874.00 EUR
Supplier

Details

Kit's description
BioVision’s Human GDF-15 (Growth Differentiation Factor 15) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GDF-15. This assay employs an antibody specific for human GDF-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and GDF-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human GDF-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDF-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. Sensitivity: 2 pg/ml. Detection Range: 2 pg/ml – 800 pg/ml. The intra-Assay reproducibility is CV
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results, strip plates and additional reagents allow for use in multiple experiments.
Kit's benefits
• Easy, convenient and reliable method to measure human GDF-15. • Detection Range: 2 pg/ml – 800 pg/ml.
Category
GDF-15 ELISA kit, GDF 15 ELISA kit
Kit's other name
Growth Differentiation Factor 15 (human) ELISA Kit
Contents
• Plate coated with anti-human GDF-15 • Wash Buffer (20x) (Item B) • Human GDF-15Standard (Item C) • Assay Diluent (Item E) (5x) • Detection Ab GDF-15 (Item F) • HRP-Streptavidin (Item G) (500x) • TMB (Item H) • Stop Solution (Item I)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
human
Applications
Quantitative protein detection, establishing normal range, validation of antibody array results, strip plates and additional reagents allow for use in multiple experiments.
Samples tested
• Serum & plasma • Cell culture supernatants • Urine
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED