Irisin Competitive ELISA Kit

BioVision’s Irisin (human) Competitive ELISA kit is to be used for the in vitro quantitative determination of human irisin in cell culture supernatants, serum and plasma. It should also work for the in vitro quantitative determination of irisin in mouse, rat and monkey biological samples. A polyclonal antibody recognizing native irisin reacts with a series of predetermined recombinant irisin standard proteins or samples under competition in the irisin-coated plate. Their relative reactivity is plotted with that of the standard proteins. Detection limit: 1 ng/ml. Note: The Limit of detection was measured by adding two standard deviations to the mean value of 50 zero standard. Assay Range: 0.001 ug/ml – 5 ug/ml. Irisin levels range in human plasma and serum from 0.2 to > 2 ug /ml.

• Detection method: Absorbance (450 nm) • Species reactivity: human, mouse, rat, monkey • Application: Quantitative ELISA

• Easy, convenient and time-saving method to assay Irisin. • The minimum detectable concentration of Irisin is 1 ng/ml. • This ELISA is specific for the measurement of natural and recombinant irisin in human samples. It should also work in mouse, rat and monkey biological samples. It does not cross-react with FNDC4, human adiponectin, human Nampt, human RBP4, human clusterin, human leptin, human vaspin, human GPX3, human resistin, human ACE2, human lipocalin-2, human ANGPTL3, human ANGPTL6, human DNER, human DLK1, human calreticulin, human IL-33, mouse Nampt, mouse clusterin, mouse vaspin, mouse resistin.

Irisin

Fibronectin Type III Domain-containing Protein 5 (cleaved) Competitive ELISA Kit , Fibronectin Type III Repeat-containing Protein 2 (cleaved) Competitive ELISA Kit, FNDC5 (cleaved) Competitive ELISA Kit

• Plate coated with Irisin Recombinant Protein (6 x 16-well strips) • Wash Buffer 10X (30 ml) • ELISA Buffer 10X (30 ml) • Detection Antibody (30 μl) • HRP 100X (HRP Conjugated anti-rabbit IgG) (150 μl) • Irisin Standard (lyophilized) (5 μg) • TMB Substrate Solution (12 ml) • Stop Solution (12 ml) • Plate sealers (plastic film)

4°C

gel pack

12 months after the day of manufacturing

Human, mouse, rat, monkey

Quantitative ELISA

• Serum & plasma • Cell culture supernatants

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED