Endothelin-1 ELISA Kit

Catalog number
K7429-100
Name
Endothelin-1 ELISA Kit
Size
100 assays
Price
985.00 EUR
Supplier

Details

Kit's description
BioVision’s Endothelin-1 (ET-1) ELISA kit is a competitive immunoassay for the quantitative determination of Endothelin-1 in biological fluids. ET-1 has identical amino acid sequence for human, mouse, rat, cow, dog, pig, and rabbit. The basal circulating level of ET-1 is reported to be < 1 to 3pg/mL but is known to be elevated in atrial and pulmonary hypertensions, atherosclerosis, congestive heart failure, cancer and variably related to lung diseases such as COPD and asthma. Samples and standards are added to wells coated with a monoclonal antibody specific for ET-1 and incubated. The plate is washed, leaving only bound ET-1 on the plate. A solution of HRP labeled monoclonal antibody to ET-1 is then added. This binds the ET-1 captured on the plate. The plate is then incubated. The plate is washed to remove excess HRP labeled antibody. TMB Substrate solution is added. The substrate generates a blue color when catalyzed by the HRP. Stop solution is added to stop the substrate reaction. The resulting yellow color is read at 450 nm. The amount of signal is directly proportional to the level of ET-1 in the sample. Sensitivity: 0.4 pg/ml (range: 0.78-100 pg/ml). The intra-Assay reproducibility is CV
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: Human, mouse, rat, cow, dog, pig and rabbit • Application: Quantitative detection of Endothelin:1
Kit's benefits
• Easy, convenient and time-saving method to measure ET-1. • Sensitivity: 0.4 pg/ml (range: 0.78-100 pg/ml).
Category
Endothelin-1 (ET-1) ELISA kit
Kit's other name
Preproendothelin-1 ELISA Kit
Contents
• Plate coated with mouse MAb to ET-1 • ET-1 Ab Concentrate • Antibody Diluent • Assay Buffer 17 • Wash Buffer Concentrate • ET-1 Standard (1000 pg/ml) • TMB Substrate • Stop Solution 2 (1 N HCl in H20) • Plate Sealer
Storage condition
4°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Human, mouse, rat, cow, dog, pig and rabbit
Applications
Quantitative detection of Endothelin-1
Samples tested
Serum, plasma, culture supernatants
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED