Colorimetric ATPase Activity Assay Kit

ATPase (Adenosine Triphosphatase: EC 3.6.1.3) is an important enzyme for maintaining the cell membrane potential, transporting ions and regulating cellular volume. It catalyzes the decomposition of ATP into ADP and a free phosphate ion. The hydrolysis of ATP is highly exergonic releasing energy that is utilized in several cellular processes. There are many classes of ATPases including Na+/K+ -ATPase, H+/K+ -ATPase, Ca2+ -ATPase, etc. The deficiency of mitochondrial ATPase is serious: for example, Na+/K+-ATPase deficiency increases anxiety-related behavior, while Ca2+ -ATPase deficiency leads to exertional muscle pain syndrome. Therefore, accurate detection of ATPase activity is valuable for the diagnosis and mechanistic studies of some of these diseases. BioVision’s ATPase Activity Assay kit provides a quick and easy method for monitoring ATPase activity in various samples. In the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, a strong, stable chromophore is generated (OD 650 nm). The assay is simple, sensitive, high -throughput adaptable and can detect ATPase Activity less than 0.005 U/L

Fast and convenient, very simple procedure: it takes less than 1 hour

Mammalian

Rapid assessment of ATPase activity in biological samples or recombinant ATPase preparations

ATPase Activity Kit

Colorimetric (OD 650 nm)

The Colorimetric ATPase Activity Assay Kit contains ATPase Assay Buffer, ATPase Substrate, ATPase Developer, Phosphate Standard (10 mM) and ATPase Positive Control

Human or animal soft tissue (i.e. brain, liver, lung, etc.) homogenates, cultured cell lysates (adherent or suspension cells), heterologously expressed recombinant ATPase preparations

ATPase Assay

Store this kit at -20°C for 12 months

This product is shipped with gel pack

Please contact us to inquire the datasheet and an example certificate of analysis

This assay kit is on the market since May 2017

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.