Human CellExp LIF, mouse recombinant

Catalog number
7268-10
Name
Human CellExp LIF, mouse recombinant
Size
10 μg
Price
445.00 EUR
Supplier
Biovision
Order

Details

Synonyms
LIF, CDF, DIA, HILDA, MLPLI
Alternates names
LIF, CDF, DIA, HILDA, MLPLI
Taglines
Affects cell growth and development
Biomolecule/Target
LIF
NCBI Gene ID #.
16878
NCBI Gene Symbol
LIF
Gene Source
Mouse
Accession #
P09056
Recombinant
Yes
Source
HEK 293 cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.3-1.2 ng/ml
Biological activity
Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.3-1.2 ng/ml
Results
N/A
Molecular Weight
Calculated MW of 20.7 kDa with no tags. The predicted N-terminus is Ser 24. DTT-reduced Protein migrates as 33-45 kDa due to different glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
Additional source
Recombinants or rec. proteins
Group
recombinants