Human CellExp HGFR/c-MET, human recombinant

Catalog number
7393-100
Name
Human CellExp HGFR/c-MET, human recombinant
Size
100 μg
Price
765.00 EUR
Supplier

Details

Synonyms
MET, AUTS9, HGFR, RCCP2, c-Met
Alternates names
MET, AUTS9, HGFR, RCCP2, c-Met
Taglines
Plays a central role in epithelial morphogenesis and cancer development.
Biomolecule/Target
HGFR/c-MET
NCBI Gene ID #.
4233
NCBI Gene Symbol
MET
Gene Source
Human
Accession #
P08581
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its binding ability in a functional ELISA Immobilized recombinant human HGF at 10 μg/ml (100 μl/well) can bind biotinylated c-Met. The EC50 of biotinylated c-Met is 2.52 μg/ml.
Results
Measured by its binding ability in a functional ELISA Immobilized recombinant human HGF at 10 μg/ml (100 μl/well) can bind biotinylated c-Met. The EC50 of biotinylated c-Met is 2.52 μg/ml.
Molecular Weight
This protein is fused with C-terminal 8×His tag. The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.5 kDa (α chain) and 60 kDa (β chain). The predicted N-terminal is Glu25 (α chain) & Ser308 (β chain). Protein migrates as 45 kDa (α chain) and 85 kDa (β chain) in reduced SDS-PAGE resulting from glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants