Human CellExp FOLR1, mouse recombinant
Catalog number
7457-50
Name
Human CellExp FOLR1, mouse recombinant
Size
50 μg
Price
765.00 EUR
Supplier
Details
Synonyms
FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha
Alternates names
FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha
Taglines
Mediates delivery of 5-methyltetrahydrofolate to the interior of cells
Biomolecule/Target
FOLR1
NCBI Gene ID #.
14275
NCBI Gene Symbol
FOLR1
Gene Source
Mouse
Accession #
P35846
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured in a competitive binding assay. When Folic Acid-Bovine Serum Albumin is immobilized at 5 μg/mL (100 μL/well), Recombinant Mouse (rm) FOLR1 inhibits 50% binding of biotinylated rm FOLR1 (7.9 nM) at the concentration range of 10-100 nM.
Results
Measured in a competitive binding assay. When Folic Acid-Bovine Serum Albumin is immobilized at 5 μg/mL (100 μL/well), Recombinant Mouse (rm) FOLR1 inhibits 50% binding of biotinylated rm FOLR1 (7.9 nM) at the concentration range of 10-100 nM.
Molecular Weight
This protein is fused with 6×his tag at the C-terminus and has a calculated MW of 25.6 kDa expressed. The predicted N-terminus is Thr 25. Protein migrates as 45-50 kDa in reduced SDS-PAGE resulting from glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
Additional source
Recombinants or rec. proteins
Group
recombinants
