Human CellExp CTLA4/CD152, human recombinant

Catalog number
7476-100
Name
Human CellExp CTLA4/CD152, human recombinant
Size
100 μg
Price
680.00 EUR
Supplier

Details

Synonyms
CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12
Alternates names
CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12
Taglines
A protein receptor that downregulates the immune system.
Biomolecule/Target
CTLA4/CD152
NCBI Gene ID #.
1493
NCBI Gene Symbol
CTLA4
Gene Source
Human
Accession #
P16410
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein (Catalog # CT4-H5229) at 2 μg/ml (100 μl/well) can bind rhB7-1/ CD80 Protein Fc Chimera (Catalog # B71-H5259) with a linear range of 0.2 - 20 ng/ml, when detected by HRP labeled Goat anti Human IgG Fc PAb. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein (Catalog # CT4-H5229) at 1 μg/ml (100 μl/well) can bind rh PD-L1 /B7-H1 /CD274 Protein Fc Chimera (Catalog # PD1-H5258) with a linear range of 20 - 200 ng/ml, when detected by HRP labeled Goat anti Human IgG Fc PAb.
Results
Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein (Catalog # CT4-H5229) at 2 μg/ml (100 μl/well) can bind rhB7-1/ CD80 Protein Fc Chimera (Catalog # B71-H5259) with a linear range of 0.2 - 20 ng/ml, when detected by HRP labeled Goat anti Human IgG Fc PAb. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein (Catalog # CT4-H5229) at 1 μg/ml (100 μl/well) can bind rh PD-L1 /B7-H1 /CD274 Protein Fc Chimera (Catalog # PD1-H5258) with a linear range of 20 - 200 ng/ml, when detected by HRP labeled Goat anti Human IgG Fc PAb.
Molecular Weight
This protein is fused with 6×his tag at the C-terminus, and has a calculated MW of 14.3 kDa. The predicted N-terminus is Ala 37. DTT-reduced Protein migrates as 25 kDa in SDS-PAGE due to glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants