Human CellExp GCSFR /CD114, human recombinant

Catalog number
7480-20
Name
Human CellExp GCSFR /CD114, human recombinant
Size
20 μg
Price
350.00 EUR
Supplier

Details

Synonyms
CSF3R, CD114, GCSFR
Alternates names
CSF3R, CD114, GCSFR
Taglines
Initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages
Biomolecule/Target
GCSFR /CD114
NCBI Gene ID #.
1441
NCBI Gene Symbol
GCSFR
Gene Source
Human
Accession #
Q99062
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured by its ability to inhibit the GCSF-induced proliferation of NFS-60 mouse myeloid cells. The ED50 for this effect is typically 0.02-2 μg /ml in the presence of 0.125 ng /ml of recombinant human GCSF.
Results
Measured by its ability to inhibit the GCSF-induced proliferation of NFS-60 mouse myeloid cells. The ED50 for this effect is typically 0.02-2 μg /ml in the presence of 0.125 ng /ml of recombinant human GCSF.
Molecular Weight
This protein is fused with 6×his tag at the N-terminus, has a calculated MW of 69 kDa expressed. The predicted N-terminus is Glu 25. Protein migrates as 94 kDa in reduced SDS-PAGE resulting from glycosylation.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants