Human CellExp IGF-1, human recombinant

Catalog number
7507-20
Name
Human CellExp IGF-1, human recombinant
Size
20 μg
Price
330.00 EUR
Supplier

Details

Synonyms
IGF-I, IGF1A, somatomedin C, MGF
Alternates names
IGF-I, IGF1A, somatomedin C, MGF
Taglines
≥98% pure, active, human cell expressed, human recombinant IGF-1/Somatomedin - a hormone similar in molecular structure to insln.
Biomolecule/Target
IGF-1
NCBI Gene ID #.
3479
NCBI Gene Symbol
IGF1
Gene Source
Human
Accession #
P05019
Recombinant
Yes
Source
HEK293 cells
Purity
N/A
Activity (Specifications/test method)
N/A
Biological activity
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.5-2.5 ng/mL.
Results
The ED50 for this effect is typically 0.5-2.5 ng/mL.
Molecular Weight
This protein rhIGFI-Fc, fused with Fc fragment of human IgG1 at the N-terminus, has a calculated MW of 35 kDa. DTT-reduced Protein migrates as 35 kDa.
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
IGF-I, IGF-II, insulin-like growth factors -1 and -2 are important regulators of signaling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades.
Additional source
Recombinants or rec. proteins
Group
recombinants