CaspSCREEN Flow Cytometric Apoptosis Detection Kit

Catalog number
K200-100
Name
CaspSCREEN Flow Cytometric Apoptosis Detection Kit
Size
100 assays
Price
565.00 EUR
Supplier

Details

Storage Conditions
-20°C
Tag Line
Measures Activated Caspases in intact cells by FACS
Shipping Conditions
gel pack
Shelf life
6-12 months
Species Reactivity
NA
Applications
NA
Sample Type
Live cells
Description
This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
Test
Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.