Target of this antibody:
Alternative names of antibody target:
FOXO4/FAX , AFX , AFX1 , MLLT7 , AFX , FoxO4 , AFX
Fox04 is a member of the Forkhead family of transcription factors which are involved in tumorigenesis and other pathological processes. Fox04 has been shown to mediate signaling via a pathway involving IGFR1, PI3K, and Akt.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 491 of human FOXO4/AFX1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-AFX polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml).However, the optimal conditions should be determined individually. 3T3 cell lysate can be used as a positive control. The antibody detects 60 kDa Fox04 from samples of human, mouse, and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.